|
?Ab Library
|
|
By Amoeba, Section Wish list
Posted on Mon Apr 28th, 2003 at 08:02:00 AM PST
|
 |
|
I propose we create a library of each possible conformation of immunoglobulin possible. It could link genetic code with functional structure, as well as the potential binding kinetics for each conformation. When one has a protein of interest they could compare it against the database to determine what Ab would represent the best fit for creating affinity columns or which Ab would target the structure of interest most effectively. Once the simple comparison had been made to the database, and the appropriate Ab selected, the database would spit out the genetic sequence for that specific immunoglobulin conformation and the researcher could then proceed to create the necessary hybridomas. The potential benefits to this are (to name a few): 1) One could control and design Ab for specific locations on the peptide / protein of interest, 2) There would ideally be a range of binding affinities to choose from which would allow the researcher to tailor the affinity column (or what have you) to their needs, and 3) Researchers would have a much more direct methodology for generating a multiple Ab cocktail for protein isolation.
|
|
|
|
|
|
|
